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B6;D2-Tg(Thy-1-SNCA)61Ema

Name: B6;D2-Tg(Thy-1-SNCA)61Ema
Description: Genetically modified mouse in which the transgene for human alpha synuclein is expressed under the control of the mouse Thy-1 promotor. The mouse was produced in the laboratory of Dr. Eliezer Masliah. * From cited reference:"For the present study, we generated tg mice expressing h-synuclein under the mThy-1 (Fig. 1A) cassette (provided by Dr. H. van der Putten, Ciba-Geigy, Basel, Switzerland)...The wt h-synuclein cDNA fragment (nt 53-475; Genbank accession No. L08850) was generated by RT-PCR from human brain mRNA, ligated into pCRII (Invitrogen, La Jolla, CA), and sequenced for accuracy. In addition, PCR-based mutagenesis was used to generate the A30P mutant h-synuclein cDNA fragment. The h-synuclein cDNA fragments were released from the pCRII vector and inserted into the mThy-1 expression cassette between exons 2 and 4 (Fig. 1A), purified, and microinjected into one-cell embryos (C57BL/6 x DBA/2F1) according to standard procedures....For the mThy-1 promoter...Mice expressing mThy-1-h-synuclein (wt; line 61) showed the highest levels of expression, equivalent to tenfold the human levels, followed by the mThy-1-synuclein (A30P) tg mice (line 44)."
URL: Rockenstein et al., Differential neuropathological alterations in transgenic mice expressing alpha-synuclein from the platelet-derived growth factor and Thy-1 promoters. J. Neursci. Res. 2002 68:568
*Id: birnlex_909
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  • Created Date: 2008-01-28

Contributors

Bandrow, Nifbot2, Smaynard



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Facts about B6;D2-Tg(Thy-1-SNCA)61EmaRDF feed
Created28 January 2008  +
CurationStatuspending final vetting  +
DefiningCitationRockenstein et al., Differential neuropathological alterations in transgenic mice expressing alpha-synuclein from the platelet-derived growth factor and Thy-1 promoters. J. Neursci. Res. 2002 68:568  +
DefinitionGenetically modified mouse in which the tr Genetically modified mouse in which the transgene for human alpha synuclein is expressed under the control of the mouse Thy-1 promotor. The mouse was produced in the laboratory of Dr. Eliezer Masliah. * From cited reference:"For the present study, we generated tg mice expressing h-synuclein under the mThy-1 (Fig. 1A) cassette (provided by Dr. H. van der Putten, Ciba-Geigy, Basel, Switzerland)...The wt h-synuclein cDNA fragment (nt 53-475; Genbank accession No. L08850) was generated by RT-PCR from human brain mRNA, ligated into pCRII (Invitrogen, La Jolla, CA), and sequenced for accuracy. In addition, PCR-based mutagenesis was used to generate the A30P mutant h-synuclein cDNA fragment. The h-synuclein cDNA fragments were released from the pCRII vector and inserted into the mThy-1 expression cassette between exons 2 and 4 (Fig. 1A), purified, and microinjected into one-cell embryos (C57BL/6 x DBA/2F1) according to standard procedures....For the mThy-1 promoter...Mice expressing mThy-1-h-synuclein (wt; line 61) showed the highest levels of expression, equivalent to tenfold the human levels, followed by the mThy-1-synuclein (A30P) tg mice (line 44)." Thy-1-synuclein (A30P) tg mice (line 44)."
Idbirnlex_909  +
LabelB6;D2-Tg(Thy-1-SNCA)61Ema  +
ModifiedDate20 September 2011  +